Aim: To extract DNA from a cultivated carrot using house hold products.
Method:
* Step 1 Pour 60 ml (¼ cup) of clear intoxicant into a nut case. Place the glass into the bowl of iced water supply to chill, then come down aside.
* Step 2 Pour 120 ml (½ cup) of strike hard/distilled water into the measuring cup with the salinity. Add 30 ml (2 tablespoons) of clear detergent and mix carefully until the salt is dissolved. This is the origin solution.
* Step 3 Grate a large carrot or 2 medium sized carrots then annex some of the extraction solution or blend the carrot with salt solution, then add the detergent. The consistency should be analogous to a thick fruit smoothie. Gently stir the resulting sort for 30-60 seconds.
* Step 4 Place a suitable leach (cloth, filter paper or a fine sieve) oer an empty glass, and secure it with the rubber band. Pour the garland into the filter. If the mixture is too thick, stir in more of the extraction solution. get by to filter for about 10 minutes.
* Step 5 close to liquid should have filtered through (the filtrate). Remove the glass of alcohol from the iced water. Gently pour the alcohol along the wall of the glass containing the filtrate. Observe after at least 5 minutes. For a closer examination, use the glass rod, popsicle stick or toothpick to hook the precipitate.
* Step 6 Pour the liquids down the fall and clean the rest of the equipment.
Read more at Suite101: How to Extract DNA From Fruit and Vegetables: A Home Experiment for think Real Deoxyribonkucleic Acid http://www.suite101.com/content/how-to-extract-dna-from-fruit-and-vegetables-a76806#ixzz1EkVq2LaJ
Theory
The mashing breaks the cells apart. The detergent breaks them open, acquittance the cell contents (including the DNA). The filtering removes most of the unwanted larger particles. The salt has positively charged ions, which stop the negatively charged DNA molecules from repelling each other. DNA is soluble in water but not alcohol, therefore the alcohol...If you want to get a full essay, order it on our website: Orderessay
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