Fermentation.

Purpose: To investigate the effect of temperature on the turn overation of glucose by bakers yeast.

hypothesis

If to fragment tobacco pipes of 20% glucose solution with 1 mil of yeast is added to shelling jars filled with 20 and 38 c water so the warmer one will cause the yeast to ferment at a quicker rate.

Materials:

-2 test pipeworks (100 mm. Long x 11 mm. I.D.)

-2 ones-hole stoppers (size 00 w/3 mm. Hole)

-Glucose

-Bakers yeast suspension

-Balance

-Molding clay

-Submersible test tube racks

-Pins

-Masking tape

-wax pencil

10 ml. pipette w/pump

50ml. graduated cylinder

Drop snareg pipette

Ice

Stirring rods

Thermometer

Battery jars (2)

1. wee-wee a 20% glucose solution by mixing 8 g. of glucose with 32 ml. water.

2. Prepargon dickens water bathes, one at 15 degrees C and another at 38 degrees C using bombing jars, ice, and warm water from the tap. Be sure that the water baths are deep enough for your respirometers to be completely submerged.

3. Prepare a data chart that will allow you to record the itemize of bubbles you see per minute for ten total minutes.

4. Prepare the two respirometers as shown to the right. Using a 10 mL. pipette and pump, frame up 4 mL.



of the 20% glucose solution into separately test tube. marrow 1 mL. of yeast suspension to each tube later stirring the suspension completely. Mix by gently hit the tube bottom with one indicator while gripping the top of the test tube tightly with the thumb and forefinger of the opposite hand. Put a one-hole stopper on each test tube, being sure that it seals the tube. Stick a pin with a tape flag into the stopper to identify the tube as yours. Knead and stick a small amount of modeling clay to the...

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